Viruses do replicate in cell-free systems

Viruses do replicate in cell-free systems.

Klingeborn et al. (1) reported a systematic study of the infectivity of transmissible spongiform encephalopathy (TSE) infectious agent generated de novo in cell-free reactions. They convincingly demonstrated the generation of new TSE infectivity in reactions primed by infected brain homogenates. Absence of infectivity in control reactions with brain homogenates lacking prion protein (PrP) suppo...

Comparative Adaptation of some pox viruses in two cell systems

Antioxidant activity of polyphenolic myricetin in vitro cell-free and cell-based systems

Myricetin (Myc) is one of the most important flavonoids in diet due to its abundance in foods with the highest antioxidant activity. The antioxidant activity of Myc was studied in cell-free and cell-based systems to evaluate the ROS protection efficiency of Myc. The studies were based on the assessment of reducing power of Myc according to ferric ion reduction and intracellular ROS level measur...

How do the ends replicate?

20 Schmeing, T.M. et al. (2005) An induced-fit mechanism to promote peptide bond formation and exclude hydrolysis of peptidyl-tRNA. Nature 438, 520–524 21 Weixlbaumer, A. et al. (2008) Insights into translational termination from the structure of RF2 bound to the ribosome. Science 322, 953–956 22 Laurberg, M. et al. (2008) Structural basis for translation termination on the 70S ribosome. Nature...

Culture in the cockpit: do Hofstede's dimensions replicate?

Survey data collected from 9,400 male commercial airline pilots in 19 countries were used in a replication study of Hofstede's indexes of national culture. The analysis that removed the constraint of item equivalence proved superior, both conceptually and empirically, to the analysis using Hofstede's items and formulae as prescribed, and rendered significant replication correlations for all ind...